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Depending on use conditions of the staining solution, a differentiation step may be required

Tekst dotyczy roztworów barwiących do zastosowań chemicznych

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https://www.ihcworld.com/_protocols/special_stains/h&e_ellis...

To ensure saturation of chemical binding sites, the stain is applied longer than necessary, resulting in the overstaining of the tissues with much non-specific background colouration. This undesirable colouration is selectively removed by controlled leaching in an alcoholic acidic solution, (acid alcohol), the process being termed "differentiation". Differentiation is arrested by returning to an alkaline environment, whereupon the haematin takes on a blue hue, the process of "blueing-up". The haematin demonstrates cell nuclei.